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anti lef1 pe  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti lef1 pe
    Anti Lef1 Pe, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lef1+pe/LEF1+Rabbit+mAb/bio_rxiv__64898__2026__01__17__700120-132-173-175
    Average 95 stars, based on 94 article reviews
    anti lef1 pe - by Bioz Stars, 2026-10
    95/100 stars

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    Article Title: TCF1 lo CD8 T cells proliferate and persist autonomously in tumors
    Article Snippet: Anti-BCL2-PE/Cyanine7 (BCL/10C4), BioLegend Cat# 633512, RRID:AB_2565247; Anti-BIM-Alexa Fluor 488 (C34C5), Cell Signaling Technology Cat# 94805, RRID:AB_2800234; Anti-BrdU-Alexa Fluor 647 (MoBu-1), Thermo Fisher Scientific Cat# B35133, RRID:AB_2536437; Anti-CD3ε-PE (145-2C11), Tonbo Biosciences Cat# 50-0031, RRID:AB_2621730; Anti-CD8a-PE/Cyanine7 (53-6.7), BioLegend Cat# 100722, RRID:AB_312761; Anti-CD8a-Brilliant Violet 711 (53-6.7), BioLegend Cat# 100748, RRID:AB_2562100; Anti-CD8a-APC/Cyanine7 (53-6.7), BioLegend Cat# 100714, RRID:AB_312753; Anti-CD25-APC-eFluor780 (PC61.5), Thermo Fisher Scientific Cat# 47-0251-80, RRID:AB_1272215; Anti-CD38-APC/Fire 750 (90), BioLegend Cat# 102738, RRID:AB_2876402; Anti-CD39-PerCP-eFluor 710 (24DMS1) Thermo Fisher Scientific Cat# 46-0391-80, RRID:AB_10717513; Anti-CD44-FITC (IM7), Tonbo Biosciences Cat# 35-0441, RRID:AB_2621688; Anti-CD62L-Brilliant Violet 785 (MEL-14), BioLegend Cat# 104440, RRID:AB_2629685; Anti-CD69-PE/Cyanine7 (H1.2F3), Thermo Fisher Scientific Cat# 25-0691-82, RRID:AB_469637; Anti-CD90.1-Brilliant Violet 510 (OX-7), BioLegend Cat# 202535, RRID:AB_2562643; Anti-CD90.1-PerCP/Cyanine5.5 (OX-7), BioLegend Cat# 202516, RRID:AB_961437; Anti-CD90.1-APC (OX-7), BioLegend Cat# 202526, RRID:AB_1595470; Anti-CD101-PE/Cyanine7 (Moushi101), Thermo Fisher Scientific Cat# 25-1011-80, RRID:AB_2573377; Anti-CD122-PE (5H4), BioLegend Cat# 105905, RRID:AB_2125737; Anti-CD127-PE/Cyanine7 (A7R34), BioLegend Cat# 135014, RRID:AB_1937265; Anti-CD132-APC (TUGm2), BioLegend Cat# 132307, RRID:AB_10643575; Anti-IFNγ-APC (XMG1.2), BioLegend Cat# 505810, RRID:AB_315404; Anti-KI67-PerCP-Cyanine5.5 (16A8), BioLegend Cat# 350520, RRID:AB_2562295; Anti-KI67-PE (16A8), BioLegend Cat# 652403, RRID:AB_2561524; Anti-KI67-APC-eFluor 780 (SolA15), Thermo Fisher Scientific Cat# 47-5698-80, RRID:AB_2688064; Anti-LEF1-PE (C12A5), Cell Signaling Technology Cat# 14440, RRID:AB_2798481; Anti-Ly108-Pacific Blue (330-AJ), BioLegend Cat# 134608, RRID:AB_2188093; Anti-PD1-PE/Cyanine (RMP1-30), BioLegend Cat# 109109, RRID:AB_572016; Anti-PD1-APC (RMP1-30), BioLegend Cat# 109112, RRID:AB_10612938; Anti-TCF1-PE (C63D9), Cell Signaling Technology Cat# 14456, RRID:AB_2798483; Anti-TCF1-Alexa Fluor 647 (C63D9), Cell Signaling Technology Cat# 6709, RRID:AB_2797631; Anti-Tim3-PE (RMT3-23), BioLegend Cat# 119703, RRID:AB_345377; Anti-TNFα-PE (MP6-XT22), Thermo Fisher Scientific Cat# 12-7321-82, RRID:AB_466199; Anti-TOX-APC (REA473), Miltenyi Biotec Cat# 130-118-474, RRID:AB_2751522.



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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases

    doi: 10.1016/j.celrep.2019.05.061

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit monoclonal anti-LEF1 (C12A5) PE , Cell Signaling , Cat#14440S; RRID:AB_1257210.

    Techniques: Virus, Plasmid Preparation, Recombinant, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Software, Extraction, Gene Expression

    Effect of maternal food restriction (MFR) on the expression of key adipogenic mRNAs in bone marrow-derived mesenchymal stem cells (BMSCs) of the resultant offspring. (A) The mRNA levels of LRP5, β-catenin, GSK3β and LEF1 showed downward trend, though it didn't reach statistical significance for GSK3β and LEF-1; in contrast the mRNA levels of LRP6 were significantly upregulated. (B) Levels of key Wnt proteins in BMSCs of the MFR offspring. The protein levels of β-catenin and LEF1 levels were down-regulated in MFR BMSCs compared to controls (N=3, *p<0.05 vs. control). (C) Expression of ZFP521 and RUNX2 proteins and mRNAs in bone marrow-derived mesenchymal stem cells (BMSCs) of the maternal food restricted (MFR) offspring. White arrows indicate the specific signals. ZFP521 staining was strong, whereas, RUNX2 staining was weaker in BMSCs derived from the MFR group compared to the control group. Consistently, mRNA expression of ZEP521 and RUNX2 show significantly reduction in BMSCs derived from the MFR group compared to the control group. (N=3, *p<0.05, ***p<0.01 vs. control).

    Journal: International journal of obesity (2005)

    Article Title: Bone Marrow Mesenchymal Stem Cells of the Intrauterine Growth Restricted Rat Offspring Exhibit Enhanced Adipogenic Phenotype

    doi: 10.1038/ijo.2016.157

    Figure Lengend Snippet: Effect of maternal food restriction (MFR) on the expression of key adipogenic mRNAs in bone marrow-derived mesenchymal stem cells (BMSCs) of the resultant offspring. (A) The mRNA levels of LRP5, β-catenin, GSK3β and LEF1 showed downward trend, though it didn't reach statistical significance for GSK3β and LEF-1; in contrast the mRNA levels of LRP6 were significantly upregulated. (B) Levels of key Wnt proteins in BMSCs of the MFR offspring. The protein levels of β-catenin and LEF1 levels were down-regulated in MFR BMSCs compared to controls (N=3, *p<0.05 vs. control). (C) Expression of ZFP521 and RUNX2 proteins and mRNAs in bone marrow-derived mesenchymal stem cells (BMSCs) of the maternal food restricted (MFR) offspring. White arrows indicate the specific signals. ZFP521 staining was strong, whereas, RUNX2 staining was weaker in BMSCs derived from the MFR group compared to the control group. Consistently, mRNA expression of ZEP521 and RUNX2 show significantly reduction in BMSCs derived from the MFR group compared to the control group. (N=3, *p<0.05, ***p<0.01 vs. control).

    Article Snippet: The specific primary antibodies used included PPARγ (H-100, Cat. No.: sc-7196, 1:200), adipocyte differentiation-related protein (ADRP) (H-80, (Cat. No.: sc-32888, 1:150), CCAAT/enhancer binding protein α (C/EBPα) (14AA, Cat. No.: sc-61, 1:200), β-catenin (E-5, Cat. No.: sc-7963, 1:250), lymphoid enhancer-binding factor 1 (LEF1) (H-70, Cat. No.: sc-28687, 1:250), All of the above primary antibodies were purchased from Santa Cruz Biotechnology, Inc, Santa Cruz, CA.

    Techniques: Expressing, Derivative Assay, Control, Staining